The main part of the Museum is constituted by the Permanent Exhibition of Cartoons, mainly carricatures, comic strips and other kinds of sketches by Greek and foreign cartoonists. There is also a library and archives.

Some of the most important exhibits of the museum are:

- Comic strips by G. Grammatopoulos: "Proteus and Andromeda", on a script by B. Rotas, from the series "Illustrated Classics" published by Atlantis,

- Caricatures of cartoonists of the early years of the 20th century, from the satyrical review "Satanas",

- Cover of the periodical "Phanos" by Phokion Dimitriades.