The Archaeological Collection of Distomo showcases finds from the ancient Phocean city of Ambrossos, which was located on the site of modern Distomo, and its broader area. The finds, which date from the Mycenaean to the Early Christian period, come primarily from rescue excavations of the Tenth Ephorate of Prehistoric and Classical Antiquities.

The collection is housed in the old Primary School, a stone building erected in 1903 and given by the town of Distomo to the Ministry of Culture in 1987. The permanent exhibition occupies two rooms and the entrance hall on the ground floor. One of the basement rooms is used as conservation laboratory for mosaics, while another houses an exhibition of photographs and other material relating to the 1944 massacre of the people of Distomo by Nazi troops.

The Archaeological Collection of Distomo is overseen by the Tenth Ephorate of Prehistoric and Classical Antiquities and attracts visitors interested in local history.